The Effect of Eight Weeks of Taking a Combined Supplement with Aerobic Exercise on Hippocampal ARC and IGF-1 Gene Expression and Cognitive Performance in Rats with Alzheimer's Disease: An Experimental Study

Document Type : Original Articles

Authors
Amir Jahanbakhsh 1
Khosro Jalali-Dehkordi 2
Farzaneh Taghian 3
1 PhD Student, Department of Exercise Physiology, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
2 Associate Professor, Department of Exercise Physiology, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
3 Professor, Department of Exercise Physiology, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
10.48305/jrrs.2023.42150.1068
Abstract
Introduction: ARC and IGF-1 are important molecular markers for brain neurogenesis, which are significantly reduced in brain tissue in various conditions such as Alzheimer's. The aim of this study was to investigate the effect of eight weeks of consuming combined supplement in conjunction with aerobic exercise on hippocampal ARC and IGF-1 gene expression and cognitive function in elderly rats with Alzheimer's disease.
Material and Methods: In this experimental study, 25 male c57 mice with Alzheimer's (age: 14-16 weeks, weight: 30-35 grams), were randomly divided into healthy control groups, Alzheimer's, Alzheimer's + aerobic exercise, Alzheimer's + combined supplement, Alzheimer's + aerobic exercise + combined supplement. Alzheimer's induction was performed by injecting amyloid beta-42 (Aβ1) into the hippocampus. The aerobic exercise protocol was five days per week for eight weeks before and after Alzheimer's induction. Kibi's supplement of resveratrol with a concentration of 25 mg/kg and fisetin with a concentration of 20 mg/kg was used for eight weeks. To analyze the data, one-way analysis of variance and Tukey's post hoc test were used at a significance level of 0.05.
Results: Alzheimer's induction caused a significant decrease in the expression of ARC and IGF-1 gene (P = 0.001), a significant increase in the expression of the APP gene in the hippocampus (P = 0.001) and a significant decrease in the cognitive function of the hippocampus (P = 0.001). Aerobic exercise and combined supplement use separately and simultaneously caused significant improvement of all the above changes (P=0.001).
Conclusion: It seems that aerobic exercise and taking a combined supplement can probably help to increase brain neurogenesis and improve cognitive function in elderly people with Alzheimer's through increasing the expression of ARC and IGF-1 in the hippocampus.
Keywords
Aerobic exercise
APP
ARC
IGF-1
Alzheimer
Subjects
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Journal of Research in Rehabilitation Sciences
Volume 19, Issue 1
April 2023
Pages 20-26