Introduction: Assessment of motor skills and the use of a suitable tool to identify children with developmental coordination disorder (DCD) appear to be critical in preschool ages. Therefore the main aim of this study was to evaluate the sensitivity and specificity of the Bruininks-Oseretsky Test of Motor Proficiency-Second Edition-Short Form (BOT-2 SF), in preschool children.Materials and Methods: A total of 306 preschool children participated in this study (4-7 years). Among them, 30 children with DCD were selected. To assess the ability of the BOT-2 SF to detect DCD, we measured the sensitivity and specificity using receiver operating characteristic (ROC) curve method.Results: The area under ROC curve for the sensitivity and specificity were 0.91 and 0.93, respectively. In general, the area under the curve was 0.97.Conclusion: The BOT-2 SF has high sensitivity and specificity in preschoolers; therefore it can be useful in evaluating motor skills and identifying children with DCD.