Journal of Research in Rehabilitation Sciences

Modulation of Muscle Atrophy in Elderly Prediabetic and Diabetic Rats through Interval Aerobic Exercise and Crocetin Extract: An Experimental Study

Document Type : Original Articles

Authors

1 PhD Student, School of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

2 Associate Professor, School of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

3 Professor, Department of Physiology, School of Sports Sciences, University of Isfahan, Isfahan, Iran

4 Professor, Department of Exercise Physiology, Marodasht Branch, Islamic Azad University, Marodasht, Iran

10.48305/jrrs.2023.41895.1059

Abstract

Introduction: MuRF1 and Foxo3 genes are important molecular markers of muscle atrophy and are significantly increased in skeletal muscle under various conditions, such as diabetes. The aim of the present study was to evaluate the modulation of muscle atrophy in elderly prediabetic and diabetic rats through interval aerobic training and crocetin extract.
Materials and Methods: In this experimental study, 45 elderly male and female c57bl6 mice with diabetes [40 mg/kg peritoneal injection of streptozotocin (STZ)] aged 14-16 weeks and weighing 30-35 grams were randomly divided into 9 healthy control, pre-diabetic, pre-diabetic + aerobic exercise, pre-diabetic + crocetin, pre-diabetic + aerobic exercise + crocetin, diabetic, diabetic + aerobic exercise, diabetic + crocetin, and diabetic + aerobic exercise + crocetin groups. Interval aerobic training was performed for eight weeks, five sessions per week. Mice received crocetin 30 mg/kg/day by peritoneal injection. MuRF1 and Foxo3 expression levels were measured by the real-time polymerase chain reaction (PCR). To analyze the data, one-way analysis of variance (ANOVA) and Tukey's post hoc test were used (P ≤ 0.05).
Results: MuRF1 and Foxo3 gene expression in the skeletal muscle of the diabetes group was significantly higher than that of the control group (P = 0.01). The gene expression levels of MuRF1 and Foxo3 in the pre-diabetes group + aerobic exercise + crocetin supplement and the diabetes group + aerobic exercise + crocetin supplement were significantly lower than those of other groups (P = 0.01). Insulin and glucose levels were significantly lower in the pre-diabetic and diabetic groups receiving aerobic exercise and a crocetin supplement than in other groups (P = 0.01).
Conclusion: It seems that interval aerobic training and crocetin, alone or synergistically, can help reduce atrophy in elderly people with prediabetes and diabetes by downregulating MuRF1 and Foxo3 in skeletal muscles.

Keywords

Main Subjects

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Journal of Research in Rehabilitation Sciences
Volume 18, Issue 1
2022