Journal of Research in Rehabilitation Sciences

Periodic Aerobic Exercise and the Active Compound Crocetin Causing Improvement in Autophagy Signaling Pathways in Cardiac Tissue of Aged Pre-Diabetic and Diabetic Mice: An Experimental Study

Document Type : Original Articles

Authors

1 PhD Student, School of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

2 Associate Professor, School of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

3 Associate Professor, Department of Sports Science, School of Literature and Humanitie, University of Qom, Qom, Iran

10.48305/jrrs.2023.41794.1055

Abstract

Introduction: Type 2 diabetes is characterized by elevated serum glucose and impaired insulin function. Exercise and crocetin supplement are known as two effective factors in preventing the complications of type 2 diabetes. The present study investigated the effects of aerobic exercise and the effective compound crocetin on the autophagy signaling pathway in a heart tissue experimental model of elderly pre-diabetic and diabetic mice.
Materials and Methods: In this experimental study, 45 elderly male and female c57bl6 mice with diabetes [40 mg/kg peritoneal injection of streptozotocin (STZ)] aged 14-16 weeks and weighing 30-35 grams were randomly divided into 9 healthy control, pre-diabetic, pre-diabetic + aerobic exercise, pre-diabetic + crocetin, pre-diabetic + aerobic exercise + crocetin, diabetic, diabetic + aerobic exercise, diabetic + crocetin, and diabetic + aerobic exercise + crocetin groups. Interval aerobic training was performed for eight weeks, five sessions per week. Mice received crocetin 30 mg/kg/day by peritoneal injection. Autophagy-related gene 5 (ATG5) and light chain 1 (LC1) expression levels were measured by real-time polymerase chain reaction (PCR). To analyze the data, one-way analysis of variance (ANOVA) and Tukey's post hoc test were used (P ≤ 0.05).
Results: The expression of ATG5 in the pre-diabetes group + aerobic exercise + crocetin supplement and the diabetes group + aerobic exercise + crocetin supplement was significantly higher than that in other groups (P = 0.01). LC1 gene expression in the pre-diabetes group + aerobic exercise + crocetin supplement and the diabetes group + aerobic exercise + crocetin supplement was significantly lower than that in other groups (P = 0.01). Moreover, the insulin and glucose levels in the pre-diabetes group + aerobic exercise + crocetin supplement and the diabetes group + aerobic exercise + crocetin supplement were significantly lower than those in the other groups (P = 0.01).
Conclusion: It seems that interval aerobics and crocetin are effective, alone and synergistically, in improving autophagy in heart tissue in pre-diabetic and diabetic mice. Therefore, the use of periodic aerobic exercise and crocetin is recommended in senile pre-diabetes and diabetes.

Keywords

Main Subjects

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Journal of Research in Rehabilitation Sciences
Volume 18, Issue 1
2022